Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR i s still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech A/S has developed the MeltPlex^® technology. 

MeltPlex^® utilizes a system of fluorescently labeled Taqman-type probes allowing each to be read out by subsequent melting curve analysis. 5 or more probes can be analyzed per fluorophore channel. By utilizing melting curve readout of modified probes – one for each target - the system adds an extra level of specificity to melting curve analysis as well as added multiplexing capacity. PCR reaction and melting analysis is performed without the need to re-open PCR reaction tubes.

MeltPlex^® gives faster results at lower cost

• Achieve single tube PCR detection of 20+ targets
• Get more answers from a single, scarce sample eg. spinal fluid samples or biopsis
• Use existing Taqman target seqences
• Can support syndromic detection - vs single agent tests