The design of TINA-PCR primers is simple, constant and reproducible.
Use your conventional primer-design method or software (e.g. Primer3) and simply order the primer with a 5´ TINA modification. At standard PCR conditions, the 5' TINA modification can be expected to increase the primer annealing temperature with 3 to 4°C.
Whether you re-order existing PCR primers with the 5´ TINA modification – or new PCR primers, there is no need for any special design considerations due to the 5´ TINA modifications. Please always redissolve lyophilized TINA primers overnight in the fridge before use to ensure that the more hydrophobic TINA modified primers are fully in dilution.
Contact our distributors to order TINA Primers.