Here you'll see the MeltPlex® multiplex PCR process
PCR primers for amplification of DNA or reverse transcribed RNA target serve as target DNA. An amplification step is performed using PCR primers and multiple target-specific MeltPlex® probes labeled with fluorescent dyes.
2. Probe cleavage
If the specific target is present, the 5’ quencher is cleaved from the probe which is now activated (fluorescent).
A second, common, quenching probe can hybridize to the Melting Tag region and generate a melting curve with a TM specific for each target-specific probes.
The detection of the activated probes is done by melting curve analysis on a Real-Time PCR system. 20+ target-specific MeltPlex® detection probes, labeled by multiple fluorophores and varying in Melting Tag temperature, enable specific detection of amplified product and the corresponding pathogen.